General Cell Based Assays
Eurofins Discoverx PathHunter eXpress UTR2 Total GPCR Internalization Assay, 10-Plate Kit. (93-0746E3CP5L)
The PathHunter UTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of UTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRM1 Total GPCR Internalization Assay, 10pl Kit. (93-0745E3CP5L)
The PathHunter OPRM1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OPRM1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged OPRM1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRM1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HCRTR2 Total GPCR Internalization Assay, 10pl Kit. (93-0730E3CP0L)
The PathHunter HCRTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress β-Catenin Nuclear Translocation Assay, 10pl Kit. (93-0743E3CP22L)
The PathHunter eXpress β-Catenin Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the Wnt-FzGSK3β pathway. This kit assesses ligand (e.g. wnt 3a) based pathway activation via detection of resulting β-Catenin nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged β-Catenin and nuclear localized EA. Upon pathway activation, induced nuclear translocation of β-Catenin leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ILEX LIFE SCIENCES LLC QuickZyme Human MMP-8 Activity Assay Kit, 96 Assays, 96-well plate format, immunoassay for active or total matrix metalloproteinase 8 (cell culture conditioned medium, serum, plasma, urine, saliva, tissue homogenates samples)
The QuickZyme Human MMP-8 Activity Assay is an enzyme activity immunoassay that enables you to specifically measure both endogenous active MMP-8 and total active MMP-8 (following activation of pro-MMP-8 on the plate with APMA). It can be used for the measurement of MMP-8 activity in various biological samples, such as cell culture conditioned medium, serum, plasma, urine, saliva and tissue homogenates. This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity. ASSAY RANGE: 10 ¿ 24000 pg/ml. SENSITIVITY: 100 pg/ml (2 h incubation); 24 pg/ml (6 h incubation); 4 pg/ml (24 h incubation). SYNONYMS: Matrix metalloproteinase-8, MMP8, Neutrophil collagenase, PMNL collagenase, PMNL-CL.
ILEX LIFE SCIENCES LLC QuickZyme Human MMP-7 Activity Assay Kit, 96 Assays, 96-well plate format, immunoassay for active or total matrix metalloproteinase 7 (conditioned culture medium, serum, urine, tissue homogenates samples)
The QuickZyme Human MMP-7 Activity Assay is an enzyme activity immunoassay that enables you to specifically measure both endogenous active MMP-7 and total active MMP-7 (following activation of pro-MMP-7 on the plate with APMA). It can be used for the measurement of MMP-7 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine. This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity. ASSAY RANGE: 0.004 ¿ 16 ng/ml. SENSITIVITY: 30 pg/ml (3 h incubation); 4 pg/ml (25 h incubation). SYNONYMS: Matrix metalloproteinase-7, MMP7, Matrilysin, Matrilysin-1, Matrin, Pump-1 protease, Uterine metalloproteinase.
Echelon Biosciences Research Labs SHUTTLE PIP KIT PI(3 4 5)P3
Echelon Biosciences - Shuttle PIP Kit - P-9039 - PI(3,4,5)P3 This item is non returnable
Abcam Adipogenesis Assay Kit (Cell-Based)
Adipogenesis Assay Kit (Cell-Based)
The product is subject to the following: Abcam Restricted Use Statement
ILEX LIFE SCIENCES LLC QuickZyme Sensitive Tissue Hydroxyproline Kit, 2 x 96 Assays, 96-well plate format, colorimetric assay for low level collagen analysis (tissue, tissue homogenates samples)
QuickZyme Sensitive Tissue Hydroxyproline Assay Kit enables collagen analysis in small tissue samples with low collagen levels. Quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. The only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of hydrolysate. No drying of hydrolysate is needed, resulting in a fast and easy (96-well plate format) assay. NOTE: Kit does not contain tubes and protocol for collagen hydrolysis. If you need these items please order the QuickZyme Sensitive Tissue Collagen Assay. Application: tissues with low collagen/hydroxyproline content (ex. liver). Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is a main component of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many studies.